Contact an Account ManagerApplication of HPLC of some antibiotics in clinical microbiology
Summary
Type: HPLC
Application #: 103480
Catalog#: 720014.40 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4x250mm
Detection: UV, 230nm
Conditions: eluent: methanol - 43mM K2HPO4 buffer, adjusted to pH 5.2 with phosphoric acid (15:85, v/v)
flow rate 1 ml/min
Authors: Knöller J
Source: J Chromatogr 427 (1988) 257 - 267
Details
Legend: Fig. 4. Chromatograms of A) buffer control spiked with 125µg/ml cefixime, B) a
blank serum (diluted 1:10 with Soerensen buffer) and C) after one month of
storage at 4°C (serum sample spiked with 250